|
KMID : 0380220070400040494
|
|
Journal of Biochemistry and Molecular Biology
2007 Volume.40 No. 4 p.494 ~ p.500
|
|
|
Molecular Identification of Four Different α-amylase Inhibitors from Baru (Dipteryx alata) Seeds with Activity Toward Insect Enzymes
|
|
Bonavides Krishna B.
Pelegrini Patricia B.
Laumann Raul A.
Grossi-de-Sa Maria F.
Bloch Carlos Jr.
Melo Jorge A.T.
Quirino Betania F.
Noronha Eliane F.
Franco Octavio L.
|
|
|
Abstract
|
|
|
|
The endophytic bruchid pest Callosobruchus maculatus causes severe damage to storage cowpea seeds, leading to economical losses. For this reason the use of α-amylase inhibitors to interfere with the pest digestion process has been an interesting alternative to control bruchids. With this aim, α-amylase inhibitors from baru seeds (Dipteryx alata) were isolated by affinity chromatographic procedures, causing enhanced inhibition of C. maculatus and Anthonomus grandis α-amylases. To attempt further purification, this fraction was applied onto a reversed-phase HPLC column, generating four peaks with remarkable inhibition toward C. maculatus α-amylases. SDS-PAGE and MALDI-ToF analysis identified major proteins of approximately 5.0, 11.0, 20.0 and 55 kDa that showed α-amylase inhibition. Results of in vivo bioassays using artificial seeds containing 1.0% (w/w) of baru crude extract revealed 40% cowpea weevil larvae mortality. These results provide evidence that several α-amylase inhibitors classes, with biotechnological potential, can be isolated from a single plant species.
|
|
|
KEYWORD
|
|
|
α-amylase inhibitors, Bean weevils, Callosobruchus maculates, Dipteryx alata
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|